| Gene name | Gene/protein annotation | Mode | Role in chemotaxis | dictyDB ID | Reference |
| act15 | Actin 15 | 1.2 | Cytoskeleton component | DDB0185015 | (1,2) |
| arpB | Polyphosphate kinase component of the Arp2/3 complex | 1.3 | Actin polymerization and polarization | DDB0185179 | (3) |
| corA | Coronin; actin modulation protein | 1.3 | Mutant has motility defects | DDB0191115 | (4) |
| arpG | p20-Arc component of the Arp2/3 complex | 1.3 | Actin polymerization and polarization | DDB0191121 | (3) |
| pkbA | Rac-alpha ser/thr Akt/PKB family kinase | 1.3 | Mutant has chemotaxis defects | DDB0191195 | (5, 6) |
| act8 | Actin 8 | 1.3 | Cytoskeleton component | DDB0216213 | (1, 2) |
| act5 | Actin 5 | 2.2 | Cytoskeleton component | DDB0220447 | (1, 2) |
| act11 | Actin 11 | 2.2 | Cytoskeleton component | DDB0220449 | (1, 2) |
| act13 | Actin 13 | 2.2 | Cytoskeleton component | DDB0220454 | (1, 2) |
| sglA | Sphingosine-1-phosphate lyase | 3.1 | Mutant has chemotaxis defects | DDB0214888 | (7) |
| carA | G-protein coupled cAMP receptor 1 | 3.3 | cAMP signaling | DDB0185024 | (8) |
| shkA | Dual-specificity protein kinase | 3.6 | Mutant has chemotaxis defects | DDB0191149 | (9) |
| rasG | Ras GTPase | 3.6 | Mutant has chemotaxis defects | DDB0201663 | (10) |
| spkA | Stress-activated MEK-like kinase | 4.3 | Mutant has chemotaxis defects | DDB0185206 | (11) |
| pteN | Phosphoinositide phosphatase alpha | 5.3 | Mutant has chemotaxis defects | DDB0191093 | (12) |
| rasC | Ras GTPase | 5.4 | Mutant has chemotaxis defects | DDB0214827 | (13) |
| regA | Cytoplasmic cAMP specific phosphodiesterase | 6.2 | Regulates PKA activity; interacts with erkB; mutant has chemotaxis defects | DDB0191479 | (14, 15) |
| acaA | Adenylyl cyclase | 6.4 | cAMP production | DDB0214814 | (16) |
| gpaB | G protein alpha-subunit 2 | 6.7 | Mutant has chemotaxis defects | DDB0191327 | (17) |
| mekA | MAP kinase kinase | 6.8 | Mutant has chemotaxis defects | DDB0191164 | (18, 19) |
| act14 | Actin 14 | 7.1 | Cytoskeleton component | DDB0220455 | (1, 2) |
| gefA | Aimless, RasGEF | 8.1 | Mutant has chemotaxis defects | DDB0191187 | (20) |
| sgcA | Guanylyl cyclase | 8.1 | Required for chemotaxis | DDB0191330 | (21) |
| erkB | Protein kinase ERK2 | 8.1 | Essential for AcaA activation | DDB0191457 | (22, 23) |
| gpbA | G protein b-subunit | 8.2 | Mutant has chemotaxis defects | DDB0185046 | (24) |
| piaA | Cytosolic regulator of adenylyl cyclase pianissimo | 8.2 | Mutant has chemotaxis defects | DDB0185055 | (25) |
| gbpC | Cyclic GMP-binding protein ser/thr kinase | 8.2 | Mutant has chemotaxis defects | DDB0191359 | (26) |
| gbpD | Cyclic GMP-binding protein | 8.5 | Mutant has chemotaxis defects | DDB0215332 | (26) |
| rasD | Ras GTPase | 8.5 | Mutant has chemotaxis defects | DDB0216195 | (13) |
| PDE3 | cGMP-specific phosphodiesterase | 15.1 | cGMP participates in chemotaxis | DDB0190048 | (27, 28) |
| trfA | Tetratricopeptide-containing transcription co-activator | 15.1 | Mutants does not aggregate | DDB0191270 | (29) |
| gefE | RasGEF E | 15.1 | Potential chemotaxis roles due to interaction with ras | DDB0191289 | (3) |
| gapA | Rho family GTPase-binding protein | 15.1 | Binds the actin bundling domain of cortexillin | DDB0191293 | (30) |
| pakA | p21-activated protein kinase | 15.1 | Required for chemotaxis in neutrophils, Dictyostelium mutant has chemotaxis defects | DDB0191313 | (3, 31) |
| gefQ | RasGEF Q | 15.1 | Potential chemotaxis roles due to interaction with ras | DDB0191356 | (3) |
| carC | G protein coupled cAMP receptor 3 | 15.1 | cAMP signaling | DDB0216181 | (8) |
| Putative Phosphatidylinositol 3-kinase | 15.1 | Phosphatidylinositols regulate chemotaxis | DDB0216895 | (28) | |
| Putative Phosphatidylinositol 4-kinase | 15.1 | Phosphatidylinositols regulate chemotaxis | DDB0218818 | (3, 32) | |
| cfaD | Countin factor associated protein | 15.1 | Mutant has reduced cAMP-stimulated ERK-2 phosphorylation | DDB0229857 | (33) |
| wasA | Wiscott-Aldrich syndrome protein | 15.1 | Controls actin polymerization; mutant has chemotaxis defects | DDB0229895 | (34) |
| culA | Cullin; F-box protein involved in protein degradation | 15.2 | Modulates PKA function through regulation of RegA protein stability | DDB0185191 | (35) |
| apm2 | AP-2 medium chain; clathrin adaptor | 15.2 | Involved in myosin II assembly | DDB0191267 | (36) |
| secG | Putative GEF, PIP3 binding protein | 15.2 | Phosphatidylinositols regulate chemotaxis | DDB0191439 | (3) |
| pipA | Phosphatidylinositol phosphodiesterase activity | 15.2 | Phosphatidylinositols regulate chemotaxis | DDB0201656 | (3) |
| pkaC | cAMP dependent protein kinase, catalytic subunit | 15.2 | Mutant fails to aggregate | DDB0215408 | (22, 37) |
| mhkA | Myosin-heavy-chain kinase A | 15.2 | Important for cell polarization | DDB0216274 | (38) |
| Similar to clathrin-associated/assembly/adaptor protein, sigma 4 | 15.3 | Clathrin regulates myosin II assembly | DDB0186249 | (36) | |
| mhcA | Myosin II heavy chain | 15.3 | Important for cell polarization in chemotaxis | DDB0191444 | (38) |
| ksnG | K7 kinesin-like protein | 15.3 | Mutant is defective in cell motility | DDB0206583 | (39) |
| acpA | Actin capping protein,CAP32 | 15.4 | Regulates actin polymerization | DDB0191202 | (40) |
| erkA | Mitogen-activated protein kinase ERK1 | 15.4 | Mutant has chemotaxis defects | DDB0201635 | (18, 19) |
| pakC | p21-activated protein kinase; putative ser/thr kinase | 15.4 | Mutant has chemotaxis defects | DDB0202066 | (41, 42) |
| racI | Rho GTPase | 15.4 | Important in neutrophil and Dictyostelium chemotaxis | DDB0214826 | (41, 43) |
| arpD | Component of the Arp2/3 complex | 15.4 | Actin polymerization and polarization | DDB0214932 | (3) |
| pkaR | cAMP-dependent protein kinase regulatory subunit | 15.4 | Mutant has chemotaxis defects; PKA regulates aggregation | DDB0214950 | (15, 22)] |
| Putative Coronin binding protein | 15.4 | Coronin is involved in motility and chemotaxis | DDB0217952 | (4) | |
| cdk8 | Cyclin-dependent kinase 8; regulator of RNA Pol II | 17.3 | Mutant fails to aggregate and to express acaA | DDB0191261 | (44) |
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